- Download Maloof lab oligo data base by csv file - Clean them up by using [oligo_position_ApE_feature_table.Rmd](https://github.com/MaloofLab/lab_oligo_database) and save the data as "Maloof_Lab_oligo.txt" - Open the file from ApE > Features > Open Feature Library - Run Features > Annotate Features using Library (it takes ~10 min depending on your machine). Note: Only perfect matched oligos will be shown up in your sequence file. - **Use your template specific primers for sequencing or PCR (eg. oJM1481 hits to two regions in pMDC32; 35S promoter region?).**