Antibiotic stocks
| name |
abb |
stock (mg/ml) |
LB plate(µg/ml) |
Vol. in 500ml LB agar (ml) |
MS plate(µg/ml) |
Vol. in 1L MS (ml) |
| ampicilin |
ap |
50 |
100 |
1 |
|
|
| kanamycin |
km/kn |
50 |
50 |
0.5 |
50 |
1 |
| spectinomycin |
sp |
100 |
100 |
0.5 |
|
|
| chloramphenicol |
cm |
34 in ethanol |
34 |
0.5 |
|
|
| rifampicin |
rif |
34 in methanol |
150 |
2.2 |
|
|
| tetracycline |
tc/tet |
12 |
12 |
0.5 |
|
|
| hygromycin |
hyg |
20 |
|
|
20 |
1 |
| carbenicillin* |
carb |
100 |
100 |
1 |
500 |
5 |
| thiamine |
carb |
50 |
|
|
50 |
1 |
| gentamycin** |
gent |
effective dose 6.5 |
15 |
0.75 |
|
|
| basta |
|
20 |
|
|
20 |
1 |
| zeocin |
Zeo |
100 |
25 |
0.125 |
|
|
- Stable substitute of Amp (recommended by Julin)
** the potency of gentamycin varies from stock to stock, the volume added must be calculated according to the potency of the stock